Biologics & Small Molecules Research

  • Measure binding affinity of purified and non-purified biological molecules
  • Rapidly perform DOEs to screen optimal assay conditions

Octet systems

Overview for Biologics & Small Molecules Research

The discovery of lead candidates during Biologics drug molecules development can be an expensive and lengthy process. The early discovery process of these molecules require researchers to select multiple candidates, confirm their mechanisms of action (MOA) against target, and investigate optimal conditions for their production and activity before looking into their downstream critical quality attributes. The lifecycle of the drug product involves target molecule identification and validation, optimal cell line development, and process development before a molecule can be deemed fit for clinical testing and manufacturing. Among label-free molecular interactions platforms, the Octet system provides researchers with unmatched ease-of-use and throughput capability in research and assay development for screening and characterization purposes. The Pioneer FE SPR instrument expands capabilities in small molecules and protein interaction studies.

  • Characterize irreversible inhibitors and measure commitment to covalency

    Characterize Irreversible Inhibitors

    The majority of small molecule inhibitor assays tested with label-free, real-time biosensor technologies are reversible interactions, characterized by commonly used kinetic rate models. However, a significant fraction of therapeutic enzyme inhibitors on the market functions through covalent modification of the target. ForteBio's Pioneer FE is a SPR platform that can be used with regenerable Streptavidin biosensors to reversibly capture protein targets and quantify the efficiency of covalent inhibitors binding to the target. The Pioneer FE system's irreversible inhibitor applications method can be used to determine inhibitor compound's commitment to covalency (Cc) as a metric for irreversible inhibitors.

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    ELISA conversion on the Octet system

    Real Time Binding Charts

    ELISA has been a common practice in the biology research laboratory since the 1970's. Despite its widespread adoption, its variability in results, susceptibility to human error, labor-intensive workflow, and slow time-to-result remain as big hurdles for users looking to adopt ELISA in high throughput applications. Faster times-to-results, better consistency, and less human intervention are the prerequisites for next generation of high throughput laboratory operation.

    • Transfer your ELISA assay onto the Octet system for real-time analysis
    • Increase precision over manual ELISA in direct quantitation assays
    • Amplify response signals in multistep quantitation assay to increase quantitation sensitivity

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  • High throughput phage display screening

    Kinetic measurement of Fab fragments

    Phage display is a technique to enable the study of protein, peptide or DNA interaction with a target protein. This molecular tool enables the discovery of high-affinity binders by using bacteriophages to present a target protein on the exterior of the viral coat, while containing the DNA encoding the target protein inside the viral coat. The resulting displaying phages can be screened for binding against a library of peptides or proteins in a high throughput fashion. QPix colony pickers can be used to automate inoculation, plating, spreading, and picking in a phage display workflow.

    Due to its high throughput design, the Octet system is routinely used as a secondary screening platform for Fab fragments and non-antibody ligands derived from phage display libraries. Using immobilized antigen, an Octet system screen can provide affinity ranking data and estimates of association and dissociation constants for each primary hit.

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    Virus and vaccine R&D on the Octet system

    Virus and vaccine R&D on the Octet system

    The trend in vaccine development is for increased capacity due to emerging new applications. Among vaccine antigens, due to structural and stability reasons, viruses and virus-like particles are particularly difficult to study. In addition to facilitating the establishment of optimal conditions for these molecules, an ideal analytical method should also be stability indicating. The Octet system is aptly suited for application in both upstream and downstream vaccine development and can be used to develop methods for potency and stability studies in addition to determining vaccine titer during manufacturing.

    • High throughput and rapid potency testing
    • Quantitate or perform rapid virus affinity characterization
    • Develop stability indicating methods
    • Study and test intact virus samples

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Resources for Biologics & Small Molecules Research

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