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AgileOptix Spinning Disk Technology

 

AgileOptix spinning disk technology

Our proprietary AgileOptix™ spinning disk technology is a microscopy innovation designed to run more complex, physiologically-relevant cellular models including 3D spheroids, tissues, live-cell assays and whole organisms. This technology combines the clarity of confocal imaging with the throughput and well-known robustness of the ImageXpress® Micro platform.

 

 

How the spinning disk technology works

One aspect of AgileOptix technology is a spinning disk light path, which includes a very bright solid state light engine. Excitation light passes through a rapidly spinning disk with symmetrically placed spirals of pinholes or slits. These pinholes or slits split the illumination light into multiple beams that scan the fluorescent sample. Emission light from the sample then passes back through the confocal disk and is then directed via a dichroic beamsplitter through the emission filter to a high-sensitivity scientific CMOS camera.

AgileOptix spinning disk confocal technology

 

The AgileOptix technology difference

Unlike other spinning disk confocal technologies used for high-content screening, AgileOptix technology offers the ability to adapt disk geometries to meet your unique assay requirements. It is one of the few technologies on the market that includes a unique dual disk configuration with both pinholes or slits to accommodate high-throughput applications.

 

AgileOptix technology enables the ImageXpress® Micro Confocal High-Content Imaging System to deliver the sensitivity and throughput needed for demanding applications by combining:

  • Powerful solid-state light engine
  • High-quantum efficiency 16-bit, >4 megapixel scientific CMOS sensor
  • >3 log dynamic range in both widefield and confocal modes
  • Large field of view (1,96 mm2 at 10X) maximizes collection of publication quality images and statistically relevant data
  • Specially designed optics to ensure the best images for your assay
  • Air objectives with up to 0,95 NA
  • Water objectives with up to 1,2 NA

 


 

Multiple confocal disk configurations for optimized assay performance

AgileOptix proprietary spinning disk technology includes simple, user-defined configurations to ensure maximum assay performance for both confocal and widefield imaging. Multiple confocal disk geometries are available, including pinhole and slit configurations.

 

 

Spinning disk geometry 60 µm pinhole
(single disk)
60 µm dual disk
with 50 µm slit
60 µm dual disk
with 42 µm pinhole
High-sensitivity detection
 
 
 
Fast acquisition
 
 
 
>3 log dynamic range†
 
 
 
Widefield mode for flat biology
 
 
 
Most confocal applications
 
 
 
Highest resolution imaging    
 
High-throughput applications  
 
 

*Powered by our highly responsive sCMOS sensor and advanced solid state light source.

 


 

Gain deeper insights with water immersion objectives

Wasserimmersionsobjektive

Water immersion objectives can offer signal increases of up to 4X** which can help scientists see deeper into 3D and thick tissue samples at lower exposure times.

  • Erhöhen Sie die Tiefe des Durchdringens von Geweben
  • Verbessern Sie die Z-Auflösung und sphärische Abweichungen
  • Erzeugen Sie schärfere, genauere Abbildungen
  • Erhalten Sie Zugang zu mehr Daten als je zuvor

Mehr erfahren  

Luft
Wasserimmersionsobjektive
Water
Wasserimmersionsobjektive

Bilder wurden bei derselben Exposition aufgenommen.

**Data and images were acquired during development using customer samples. Die Ergebnisse können abweichen.


*High-performance 3D customizations

AgileOptix technology offers the flexibility to add high-performance customizations. Water immersion objectives, combined with our custom deep tissue confocal disk and high-intensity lasers described below, allow you to penetrate even deeper into your 3D samples and capture sharper, crisper images.

Molecular Devices can successfully tailor the ImageXpress Micro Confocal system to include customized software and hardware including the features described herein, as well as integration of other lab components such as incubators, liquid handlers, and robotics for a fully automated workcell. Aufgrund unserer über 30 Jahre Erfahrung in der Life-Science-Branche können Sie sich darauf verlassen, dass wir Qualitätsprodukte liefern und weltweite Unterstützung bieten.
 
Der Vertrieb unterliegt unseren Kaufbedingungen für benutzerdefinierte Produkte, einzusehen unter www.moleculardevices.com/custom-products-purchase-terms

 

Laser mit höchster intensität

High output laser excitation can reduce exposure times by up to 75%.** The laser light source is available as either a 5-channel or 7-channel with outputs of 400 —1.000 mW/channel. The 7-channel laser light source Includes near IR and is ideal for customers with increased multiplexing requirements.

  • Erhalten Sie schärfere Bilder mit größerem Signal-Rausch-Verhältnis
  • Generate up to a 2X** boost in scan speed attributed to significantly reduced exposure times
  • Führen Sie FRET-Experimente mit Lasern für CFP und YFP durch
Standard
Abbildungen bei standarintensität
Laser
Laser mit höchster intensität

Bilder wurden bei derselben Exposition aufgenommen.

**Data and images were acquired during development using customer samples. Die Ergebnisse können abweichen. Der Preis der hervorgehobenen Funktionen, die Lieferzeit und die Spezifikationen variieren je nach beiderseits abgesprochenen technischen Anforderungen. Die Lösungsanforderungen können zu Anpassungen der Standardleistung führen.

Deep Tissue Penetrating, Confocal Disk Module

An upgrade to the deep tissue penetrating, confocal disk module, combined with a laser light source, can improve resolution when imaging thick tissue samples.**

  • Verbessern Sie die Unterdrückung von Licht außerhalb des Fokus
  • Verringern Sie Eintrübungen (Pinhole-Crosstalk)
  • Dringen Sie tiefer in dicke Gewebe vor – für schärfere Bilder
Standard Spinning-Disk
Deep tissue penetrating, confocal tissue module
Deep tissue penetrating, confocal tissue module
Confocal Tissue Module

Bilder wurden bei derselben Exposition aufgenommen.

**Data and images were acquired during development using customer samples. Die Ergebnisse können abweichen. Der Preis der hervorgehobenen Funktionen, die Lieferzeit und die Spezifikationen variieren je nach beiderseits abgesprochenen technischen Anforderungen. Die Lösungsanforderungen können zu Anpassungen der Standardleistung führen.

Resources for AgileOptix Spinning Disk Technology

Videos und Webinare

Wasserimmersionsobjektive & High-Content-Imaging

Gewinnen Sie mit Wasserimmersionsobjektiven tiefere Einblicke in 3D-Zellstrukturen