Skip to main content
Molecular Devices, LLC
  •   美谷分子仪器(上海)有限公司
    咨询服务热线:400-821-3787
  •   0 Cart
  • Store

  •  Karriere
  •  Sprache  
    • English
    • Deutsch
    • 中文
    • 日本語
    • Français
    • 한국어
    • Italiano
    • Español
Molecular Devices, LLC
  • Produkte
    • Produkte

      Die neue Technologie des DispenCell™ Single-Cell Dispenser isoliert einzelne Zelllinien 3-mal schneller und mit geringeren Kosten

      • Einfache, intuitive Konfiguration
      • Sofortiger Nachweis der Klonalität und Zurückverfolgbarkeit
      • Die einzigartige Technologie handhabt die Zellprobe schonend
      • Labortisch-Größe  
        entwickelt
      • Eine patentierte  
        Einwegspitze
      Die Technologie des DispenCell™ Single-Cell Dispenser

      Automatisierte High-Content-Screening-Lösung mit dem 6-Achsen-Roboterarm des BioAssemblyBot – für optimierte 3D-Gewebe- und Organoid-Workflows

      6-Achsen-Roboterarm des BioAssemblyBot

    • Mikroplatten-Reader
      SpectraMax Mini Multi-Mode Mikroplatten- Reader
      Multi-Mode-Reader
      • SpectraMax i3x
      • SpectraMax iD3 / iD5
      • SpectraMax M Serie
      • FlexStation 3
      • SpectraMax Mini
      Spectramax ABS Mikroplatte
      Absorptions-Reader
      • SpectraMax ABS / ABS Plus
      • SpectraMax VersaMax
      • SpectraMax QuickDrop
      • CMax Plus
      Fluoreszenz-Reader
      Fluoreszenz-Reader
      • SpectraMax Gemini
      SpectraMax Lumineszenz
      Lumineszenz-Reader
      • SpectraMax L

       

      MultiWash+ Mikroplatten-Washer
      Mikroplatten-Stacker und -Wascher
      • StakMax Microplate Handler
      • Aquamax Wascher
      • MultiWash+ Mikroplatten-Washer
      • MultiWash–C 微孔板洗板机
      SoftMax Pro Datenerfassung
      Software für Mikroplatten
      • SoftMax Pro Software
      • SoftMax Pro GxP Software
      GxP-Lösungen
      GxP-Compliance-Lösungen
      • SoftMax Pro GxP Software
      • Software-Installations- und -Validierungsservice
      • IQ/OQ/PM-Service
      • SpectraTest Validierungsplatten
      Labor-Automatisierung und kundenspezifische Anpassung
      Labor-Automatisierung und kundenspezifische Anpassung
      • Laborautomatisierung für plattenbasierte Hochdurchsatz-Assays
    • Zelluläre Imaging-Systeme
      ImageXpress Pico automatisiertes Zell-Imaging-System
      Automatisierte Zell-Imaging-Systeme
      • ImageXpress Pico
      • ImageXpress Nano
      High-Content-Imaging
      High-Content-Imaging
      • ImageXpress Confocal HT.ai
      • ImageXpress Micro Confocal
      • ImageXpress Micro 4
      Stratominer-Analyse
      Datenerfassungs- und Datenanalyse-Software
      • IN Carta
      • StratoMineR
      • MetaXpress
      • CellReporterXpress
      • MetaMorph
      Labor-Automatisierung und kundenspezifische Anpassung
      Labor-Automatisierung und kundenspezifische Anpassung
      • Labor-Automatisierung für das High-Content-Screening (HCS) im High-Throughput-Format
      • BioAssemblyBot 400 Bioprinter Automatisierte HCS-Lösung
    • Klon-Screening
      Clone Pix Serie
      Picken von Säugetierzell-Kolonien
      • Clonepix 2
      QPix Microbial Colony Picker
      Picken von Mikrobenkolonien
      • QPix 420
      • QPix 450/460
      • QPix HT
      Cloneselect Imager FL
      Einzelzell-Imaging
      • CloneSelect Imager
      • CloneSelect Imager FL
      DispenCell Single-Cell Dispenser
      Einzelzell-Isolierung
      • DispenCell Single-Cell Dispenser
      Labor-Automatisierung
      Labor-Automatisierung und kundenspezifische Anpassung
      • Labor-Automatisierung für das High–Throughput-Screening von Klonen
      CloneMedia und XP Media
      Zellkulturmedien und Reagenzien
      Clone Screening Assay kit
      Clone Screening Assay kit
    • Flipr Penta
      Flipr Penta
      FLIPR Penta
      • FLIPR Penta High-Throughput Cellular Screening System
      ScreenWorks
      ANALYSE-SOFTWARE
      • ScreenWorks Software
      • Peak Pro 2 Software Module
      Flipr Assay-Kit
      FLIPR Assay-Kits
      • Calcium-Assay-Kits
      • Kalium-Assay-Kit
      • Membranpotenzial-Assay-Kit
      • EarlyTox Kardiotoxizitäts-Kit
    • Axon Patch-Clamp
      Transparent
      Verstärker
      • Axopatch 200B Capacitor
      • MultiClamp 700B
      • Axoclamp 900A
      Transparent
      Digitalisierer
      • Axon Digidata 1550B Low
      Transparent
      Datenerfassungs- &
      Datenanalyse-Software
      • pCLAMP 11 Software-Suite
    • Weitere Produkte
      Threshold Immunoassay-System
      Threshold Immunoassay-System
      GenePix Microarray-Scanner
      GenePix Microarray Scanner
      Imagexpress Micro Xls
      ImageXpress Micro XLS
      Zertifiziert Generalüberholt
      Zertifiziert Generalüberholt
      IDB-Lösungen
      F&E Cloud-Lösungenvon IDBS
    • Assay-Kits
      CardioTox
      • EarlyTox Kardiotoxizitäts-Kit
      Zellüberlebensfähigkeit
      • EarlyTox Zellintegritätskit
      • EarlyTox Zellviabilitäts-Assay-Kits
      dna quantitation
      • SpectraMax Quant dsDNA Assay-Kits
      ELISA, Western Blot
      • Catchpoint SimpleStep Elisa-Kits
      • ScanLater Western Blot-Assay-Kit
      GPCR
      • FLIPR Calcium-Assay-Kits
      • Fura-2 QBT Calcium-Kit
      • CatchPoint cAMP-Fluoreszenz-Assay-Kit
      • CatchPoint cGMP Fluoreszenz-Assay-Kit
      Ionenkanal
      • FLIPR Kalium-Assay-Kit
      • FLIPR Membranpotenzial-Assay-Kits
      IGG-QUANTIFIZIERUNG
      • ValitaTiter
      • CloneDetect
      Reportergen
      • SpectraMax Glo Steady-Luc Reporter Assay-Kit
      • SpectraMax DuoLuc Reporter Assay-Kit
      TRANSPORTER
      • QBT Fettsäure-Aufnahme-Assay-Kit
      • Neurotransmitter-Transporter-Aufnahme-Assay-Kit
      Weitere
      • Assays zur Erkennung von Kontaminierungen
      • Enzyme – IMAP-Assays
    • Zubehör und Verbrauchs­materialien
      Mikroplatten-Reader
      • 384-Well SBS
      • 384-Well-Platte für hohe Probenrückgewinnung
      • Deep-Well-Platten
      • Niedrigprofil-Mikroplatten
      • SpectraDrop Mikrovolumen-Mikroplatte
      • SpectraMax Injektionskartusche mit SmartInject Technologie
      • SpectraMax MiniMax 300 Imaging-Zytometer
      • Western Blot-Kartusche
      • 96孔微孔板
      Klon-Screening
      • Anpassbare Petrischalen- und Mikroplattenhalter
      • Bioassay QTrays
      • CaliBeads
      • Folien und Deckel
      • Chroma-Filter
      • Reinigungs- und Sterilisationslösungen
      • CloneSelect Single-Cell Printer Kartuschen
      • QPix Nadeln und Köpfe
      • QReps Replikatoren
      Axon Patch-Clamp
      • SoftPanel Verstärker-Steuerungseinheit
      Spectra Img
  • Anwendungen
    • ANWENDUNGEN

      Molecular Devices erwirbt mit der Übernahme von Cellesce die firmeneigene Technologie für von Patienten stammende Organoide

      6. Dez. 2022

      • Die erste Technologie ihrer Art von Cellesce erzeugt konsistente, von Patienten stammende Organoide für das Screening großer Wirkstoffe
      • Akquisition stärkt Position von Molecular Devices als Innovator von 3D-Biologielösungen
      • Die kombinierte Expertise wird die Akzeptanz physiologisch relevanter Zellmodelle in der Wirkstoffforschung in der Industrie beschleunigen

       

      OIC besuchen

      Pressemitteilung lesen

      Zellstoff
      Spectra für Anwendungen
    • SARS-CoV-2 (COVID-19)
      Covid-19
      COVID-19 Forschungslösungen
      Covid-19
      COVID-19 Unternehmensupdates
      Covid-19
      Arbeitsabläufe Impfstoffentwicklung
      Anwendung für die Erforschung von Infektionskrankheiten
      Anwendungen für die Impfstoffforschung
    • Forschungsgebiete
      Transparent
      3D-Zellmodelle
      Transparent
      Krebsforschungs-Lösungen
      Transparent
      Zelllinienentwicklung
      Transparent
      Wirkstoffforschung und Wirkstoffentwicklung
      Transparent
      Lebensmittel und Getränke
      Transparent
      Gen Editing (CRISPR/Cas9)
      Transparent
      Organoid-Forschung
      Transparent
      Stammzellforschung
      Transparent
      Toxikologie
    • Mikroplatten-Reader
      Transparent
      Zellgesundheit
      Transparent
      Zelluläre Signalgebung
      Transparent
      ELISA
      Transparent
      Mikrobiologie und Kontaminationen
      Transparent
      NACHWEIS & ANALYSE VON NUKLEINSÄURE (DNA/RNA)
      Transparent
      Nachweis, Quantifizierung, Analyse von Proteinen
      Transparent
      Technologie: Detektionsmodi
      • Absorption
      • Fluoreszenz
      • Fluoreszenz-
        polarisation
      • Lumineszenz
      • TRF, TR-FRET und HTRF
      • Western Blot
    • Zelluläre Imaging-Systeme
      Transparent
      Zellzählung
      Transparent
      Zell-Imaging & Analyse
      Transparent
      Zellmigrations-Assays
      Transparent
      Cell Painting
      Transparent
      Lebendzellen-Imaging
      Transparent
      Neuritenauswuchs
      Transparent
      Organ-on-a-Chip
      Transparent
      Organoide
      Transparent
      Sphäroide
    • Klon-Screening
      Transparent
      Arbeitsablauf zur Entwicklung einer Zelllinie
      Transparent
      Monoklonale Antikörper (mAbs)
      • Hybridom
      • Phage Display
      • Herstellung monoklonaler Antikörper
      Transparent
      Monoklonalität
      Transparent
      Synthetische Biologie
    • Flipr Penta
      Transparent
      GPCRs (G-Protein-gekoppelte Rezeptoren)
      Transparent
      Ionenkanäle
      Transparent
      Kardiotoxizität
    • Axon Patch-Clamp
      Transparent
      Patch-Clamp Elektrophysiologie
  • Ressourcen
    • Ressourcen
    • Ressourcen-Zentrum
      Menü Ressourcensymbole / Application Notes
      Application Notes
      Menü Ressourcensymbole / Quellennachweise
      Quellennachweise
      E-Book-Icon
      E-Books
      Menü Ressourcensymbole / wissenschaftliche Poster
      Wissenschaftliche Poster
      Menü Ressourcensymbole / Tutorials und Videos
      Videos und Webinare

      Suche

    • Blog – Lab Notes
      Spacer
      Eine Fallstudie zu Assay-...
      Spacer
      3D organoids and…
      How 3D Cell Models Will Shape the Future of Drug Discovery
      7. Mär. 2023 Target discovery and drug development rely heavily on 2D cell and animal models to decipher efficacy and toxic effect of drug candidates. Yet, 90% of candidates fail to…
      Mehr erfahren  
    • Bahnbrechende Kundenerfolge

      Erfahren Sie, wie Wissenschaftler mit unseren Produkten und Lösungen Ihre Forschung voranbringen.

    • Innovationen
      Transparent
      KI, MASCHINELLES LERNEN & DEEP LEARNING
      Transparent
      AgileOptix™ Spinning-Disk-Technologie
      Transparent
      Autofokus
      Transparent
      Digitale konfokale Option
      Transparent
      High-Content-Screening
      Transparent
      HumSilencer Technologie
      Transparent
      Laserbeleuchtung
      Transparent
      QuickID gezielte Bildaufnahme
    • Technologie
      Transparent
      Absorption
      Transparent
      Elektrophysiologie
      Transparent
      Fluoreszenz
      Transparent
      Fluoreszenz- Polarisation (FP)
      Transparent
      Lumineszenz
      Transparent
      TRF, TR-FRET und HTRF
      Transparent
      Wasserimmersionsobjektive
      Transparent
      Western Blot
    • Video-Galerie
      Transparent
      Mikroplatten-Reader
      Transparent
      Zelluläre Imaging-Systeme
      Transparent
      FLIPR-System
      Transparent
      Klon-Screening
      Transparent
      Axon Patch-Clamp
      Transparent
      On-demand-Webinar
  • Service und Support
    • Service und Support
    • Überblick
      Spectra-Logo
      SPECTRANETKundenbetreuungs-Portal
      GxP-Konformität
      GxP-COMPLIANCE-LÖSUNG
      Labor-Automatisierung und kundenspezifische Anpassung
      LABOR-AUTOMATISIERUNG UND BENUTZERDEFINIERTE ANPASSUNG
      Professionelle Serviceleistungen
      PROFESSIONELLE SERVICELEISTUNGEN

      TECHNISCHER KUNDENDIENST

      NORDAMERIKA  
      +1 800-635-5577  
      Mo bis Fr, 7 Uhr – 17 Uhr PST

      EUROPA  
      +44-118-944-8000  
      Mo bis Fr, 8 Uhr – 17 Uhr GMT

      Regionale Unterstützung finden  

      Spectranet Kunden-Portal  

    • Kunden-Portal – Spectranet
      Spectranet

      INTRODUCING OUR NEW CUSTOMERCARE PORTAL

      SpectraNet is an intuitive, simple-to-use, self-service customer portal providing a new level of experience available 24/7.

      Create your account today to get full access to integrated content and world-class customer service.

      REGISTER LOGIN
      Support-Ticket erstellen
      Erstellen Sie ein Support-Ticket
      Wissensbasis
      Wissensbasis
      Produkt registrieren
      Registrieren Sie Ihr Produkt
      Technische Ressourcen
      Technische Ressourcen
    • GXP-COMPLIANCE-LÖSUNGEN
      GxP Softmax Pro GxP Software
      SOFTMAX PRO GXP SOFTWARE
      Installation der GxP Software
      SOFTWARE-INSTALLATIONS- & -VALIDIERUNGSSERVICE
      GxP Spectratest Validation Plates Rezertifizierung
      SPECTRATEST VALIDIERUNGSPLATTEN
      IQ/OQ-Service
      IQ/OQ/PM-SERVICE
    • Labor-Automatisierung
      Labor-Automatisierung und kundenspezifische Anpassung
      Labor-Automatisierung und kundenspezifische Anpassung
      High-Content-Screening HCS
      HIGH-THROUGHPUT-HIGH-CONTENT-SCREENING
      • BioAssemblyBot 400 Bioprinter Automatisierte HCS-Lösung
      Plattenbasierte Assays mit hohem Durchsatz
      Plattenbasierte High-Throughput-Assays
      High-Throughput-Screening von Klonen
      High-Throughput-Screening von Klonen
    • PROFESSIONELLE SERVICELEISTUNGEN
      Professionelle Serviceleistungen
      PROFESSIONELLE SERVICELEISTUNGEN
      Servicepläne
      SERVICEPLÄNE
  • Unternehmen
    • Unternehmen

      MOLECULAR DEVICES BAUT GLOBALES F&E-ZENTRUM IN ÖSTERREICH AUS

      12. Okt. 2022        
      Ein größerer Standort wird in Zukunft das Organoid Innovation Center Salzburg beherbergen – ein Ort der Zusammenarbeit für die Weiterentwicklung der automatisierten Zelllinienentwicklung, Organoidentwicklung und von Screening-Lösungen zur Verbesserung der Wirkstoffforschung.

       

      OIC besuchen

      Pressemitteilung lesen

      Bandzeremonie des österreichischen Forschungs- und Entwicklungszentrums
      Spectra für Anwendungen
    • Über uns

      Wir versorgen unsere Kunden seit 40 Jahren mit innovativen bioanalytischen Lösungen für die Protein- und Zellbiologie.

    • Führungsteam

      Unsere breitgefächerten Erfahrungen, unsere Branchenkenntnis und unsere gemeinsame Zielsetzung leiten uns in unseren täglichen Entscheidungsprozessen und ermutigen unsere Mitarbeiter, Ihr Potential maximal zu entfalten.

      Führungsteam

    • Karriere

      Unsere teamorientierte Unternehmenskultur garantiert eine große Vielfalt an Denkweisen und Perspektiven, und eine starke Vertrauensbindung.

    • Pressemitteilungen
      Transparent
      NEUIGKEITEN
      Transparent
      IN DEN NACHRICHTEN
      Silver Sponsor Molecular Devices at Society for Laboratory Automation and Screening 2023 International Conference and Exhibition
      Feb 22, 2023 Showcasing new industry collaborations, automated technology, and workflow innovations that span 3D biology, cell line development, and drug…
      Read more  
    • Ereignisse
      Focus on Microscopy (FOM)
      Conference | Europe | Porto, Portugal, Europe– Apr 02 – Apr 5, 2023 FOM2023 continues a long-standing (since 1988), yearly conference series on the latest innovations and developments in (optical) microscopy and their…
      Read more  
      Solutions in Cell Line Development
      User Meeting | Europe | Trieste, Italy– Apr 19, 2023 We are pleased to announce our workshop on cell line development solutions, the Molecular Devices team for the Biopharma unit with our loyal…
      Read more  
  • Kontaktieren Sie uns
  • Suche

    Suche

  • Angebot anfordern

Mobile navigation

  • Products
    • All Products
    • Microplate readers
      • Multi-Mode Readers
      • Absorbance Readers
      • Fluorescence Readers
      • Luminescence Readers
      • Microplate Stacker
      • Microplate Washers
      • SoftMax Pro Software
      • SoftMax Pro GxP Software
      • GxP Compliance Solutions
      • Lab Automation & Customization
    • Cellular Imaging Systems
      • Automated Cell Imaging Systems
      • High-Content Imaging
      • Acquisition & Analysis Software
      • Lab Automation & Customization
    • Clone Screening
      • Mammalian Colony Picking
      • Microbial Colony Picking
      • Single-Cell Imaging
      • Single-cell Isolation
      • Lab Automation & Customization
      • Culture Media & Reagents
      • Clone Screening Assay kit
    • FLIPR Penta
      • FLIPR Penta
      • ANALYSIS SOFTWARE
      • FLIPR ASSAY KITS
    • Axon Patch-Clamp
      • Amplifiers
      • Digitizers
      • pCLAMP Software
    • Additional Products
      • Threshold Immunoassay System
      • GenePix Microarray Systems
      • ImageXpress Micro XLS
      • Certified Refurbished
      • R&D CLOUD SOLUTIONS BY IDBS
    • Assay Kits
      • Cardiotox
      • CELL VIABILITY
      • DNA QUANTITATION
      • ELISA, WESTERN BLOT
      • GPCR
      • ION CHANNEL
      • IgG quantitation
      • REPORTER GENE
      • Transporters
      • OTHER
    • Accessories and Consumables
      • MICROPLATE READERS
      • CLONE SCREENING
      • Axon Patch-Clamp
  • Applications
    • All Applications
    • SARS-CoV-2 (COVID-19)
      • COVID-19 Research Solution
      • COVID-19 Company Update
      • Vaccine Development Workflows
      • Vaccine Research Applications
    • Research Areas
      • 3D Cell Models
      • Cancer Research Solutions
      • Cell Line Development
      • Drug Discovery and Development
      • Food & Beverage
      • Gene Editing (CRISPR/Cas9)
      • Organoid Innovation Center
      • Stem Cell Research
      • Toxicology
    • Microplate Readers
      • Cell Health
      • Cellular Signaling
      • ELISA
      • Microbiology & Contaminants
      • NUCLEIC ACID (DNA/RNA) DETECTION & ANALYSIS
      • PROTEIN DETECTION, QUANTITATION, ANALYSIS
      • Technology: Detection modes
        • Absorbance
        • Fluorescence
        • Fluorescence Polarization
        • Luminescence
        • TRF, TR-FRET & HTRF
        • Western Blot
    • Cellular Imaging Systems
      • Cell Counting
      • Cell Imaging & Analysis
      • Cell Migration Assays
      • Cell Painting
      • Live Cell Imaging
      • Neurite Outgrowth
      • Organ-on-a-chip
      • Organoids
      • Spheroids
    • Clone Screening
      • Monoclonal Antibodies (mAbs)
      • Monoclonality
      • Synthetic Biology
    • Flipr Penta
      • GPCRs (G protein-coupled receptors)
      • Ion Channels
      • Cardiotoxicity
    • Axon Patch-Clamp
      • Patch Clamp Electrophysiology
  • Resources
    • Resource Hub
      • Application Notes
      • Citations
      • eBooks
      • Scientific Posters
      • Videos & Webinars
    • Blog–Lab Notes
    • Customer Breakthroughs
    • Innovations
      • AI, MACHINE LEARNING & DEEP LEARNING
      • AgileOptix Spinning Disk Technology
      • Autofocus
      • Digital Confocal Option
      • High Content Screening
      • HumSilencer Technology
      • Laser Illumination
      • QuickID Targeted Image Acquisition
    • Technology
      • Absorbance
      • Electrophysiology
      • Fluorescence
      • Fluorescence Polarization (FP)
      • Luminescence
      • TRF, TR-FRET & HTRF
      • Water Immersion Objectives
      • Western Blot
    • Video Gallery
      • Microplate Readers
      • Cellular Imaging Systems
      • Flipr System
      • Clone Screening
      • Axon Patch-Clamp
      • On-Demand Webinar
  • Service & Support
    • Overview
      • SPECTRANET Customer Care Portal
      • GXP COMPLIANCE SOLUTION
      • LABORATORY AUTOMATION SOLUTIONS
      • PROFESSIONAL SERVICES
    • Customer Portal - Spectranet
      • REGISTER
      • LOGIN
      • File a support ticket
      • Knowledge base
      • Register your product
      • Technical resources
    • GXP COMPLIANCE SOLUTIONS
      • SOFTMAX PRO GXP SOFTWARE
      • Software Installation and Validation
      • SpectraTest Validation Plates
      • IQ/OQ/PM SERVICES
    • LAB AUTOMATION
      • Lab Automation & Customization
      • HIGH-THROUGHPUT, HIGH CONTENT SCREENING
      • High-throughput Plate-Based Assays
      • High-throughput Clone Screening
    • Professional Services
      • PROFESSIONAL SERVICES
      • Service Plans
  • Company
    • About Us
    • Leadership
    • Careers
    • Newsroom
      • News
      • In The News
    • Events
  • Contact Us
  • Request Quote
  1. Home
  2. Lab Notes
  3. Cellular Imaging Systems
  4. 3D organoids and automation of complex cell assays [Podcast]
Molecular Devices Lab Notes

3D organoids and automation of complex cell assays [Podcast]

  • January 27, 2023
  • Oksana Sirenko, PhD

As we enter the era of sophisticated drug discovery with gene therapy and personalized medicine, we need to be prepared to study complex diseases, assess the therapeutic effect of drugs and identify adverse effects that can pose risks to patient health. Unfortunately, the current preclinical methods, such as animal models or 2D cell cultures, are inadequate. Because the physical and chemical properties of these models do not represent the human condition, preclinical drug evaluation does not translate to clinical success. That’s why the development of 3D cell models, such as organoids, can be a huge milestone for improving the evaluation of drug efficacy and safety.

Dr. Oksana Sirenko is the senior manager of assay development at Molecular Devices, working on the development of complex cell-based models for 3D biology, as well as high-content imaging and assay automation.

Dr. Oksana Sirenko Senior scientist

After developing high-throughput cell-based assays at several biotechnology companies, such as Bayer, Fibrogen, and Bioseek, she became part of the Molecular Devices team as a research scientist in 2009. She is responsible for developing and optimizing stem cell-derived 3D models for anti-cancer drug screening, as well as drug toxicity assessment for neurons, the cardiovascular system, and the liver. Oksana holds a PhD in Biochemistry/Biophysics, has over 15 years of industry experience, and has authored over 35 scientific papers.

In this podcast excerpt, Senior scientist Oksana discusses the advantages of 3D cell models while addressing challenges in 3D cell imaging, such as image quality, high throughput, automation, and analysis.

Table of Contents

  1. Why are 3D cell models and 3D organoids so useful in disease research and drug screening?
  2. Why does the complexity of 3D models present a hurdle/challenge for researchers?
  3. Can you outline a typical workflow for 3D organoid development and analysis?
  4. Can you tell me a bit about how you apply 3D cell model workflows specifically to your research?
  5. Can you explain how to automate the workflow for the development and analysis of organoids?
  6. How can automation aid in the research of complex systems specifically?
  7. How will you use these systems again in your future research?
  8. How will the automation of 3D organoid analysis evolve in the future?

1. Why are 3D cell models and 3D organoids so useful in disease research and drug screening?

The main problem in current disease research and drug development is that only about 3% of developed drugs make it to the clinic. The majority of drugs fail in clinical trials because of a lack of efficacy or unwanted toxicity problems. Better assay systems and disease models are needed to facilitate drug discovery and better predict success in the clinic.

Today, biology is shifting toward increased complexity for assays and models that can be used for drug discovery and development. 3D models are believed to bridge the gap between traditional cell-based models, and tissues and organs. 3D models, which include spheroids, organoids, and organ-on-a-chip, present a variety of human cell types, such as liver, immune cells, cardiac cells, and fibroblasts. Also, they can mimic the morphology of human tissue types, such as 3D tumor growth, crypts in intestinal organoids, neural tubes, or the flow of liquids. Finally, they represent at least some aspects of tissue functionality, from the metabolic activity of the liver to the beating of cardiac organoids to spikes of neuronal activity in brain organoids. This greater complexity and sophistication allow us to mimic processes in tissues, interactions between cell types, responses to drugs, toxicity effects, and processes of drug penetration into the tissue.

3D triple-negative breast cancer patient-derived tumoroids

Cerebral organoids show organization reminiscent of a developing brain.

2. Why does the complexity of 3D models present a hurdle/challenge for researchers?

Traditional 2D cell assays are easier to work with, but 3D assays have greater predictability and allow the generation of more biologically relevant data. However, despite the increasing interest in 3D research, the wide adoption of assays is limited by technical hurdles and assay complexity, which leads to higher costs, lower throughput, and a lack of reproducibility. Greater complexity presents challenges, but opportunities for instrument development and automation development would enable scientists to run 3D assays with greater throughput and accuracy.

3. Can you outline a typical workflow for 3D organoid development and analysis?

The typical workflow for organoids assays contains a number of steps, and this process is typically much longer than 2D workflow steps.

Organoids workflow illustration

3D organoids can be derived from primary cells like intestinal organoids or induced pluripotent stem cells (iPSCs), e.g., neuronal or cardiac organoids. The workflow may start from 2D pre-culture or expansion of iPSC cells, followed by the differentiation step. After that, cells are mixed with Matrigel, and typically developed inside Matrigel domes, which also may include passaging and expansion. Intestinal organoids, colorectal, pancreatic, and liver are typically developed using this Matrigel dome step.

Alternatively, some other organoid types do not require Matrigel but instead are developed in low attachment plates (e.g., cardiac organoids).

Development of organoids takes from a few days to several weeks. Some protocols even require a few months. This is a very tedious process and will greatly benefit from automation.

Finally, the endpoint assay, whether it is a drug treatment, viral infectivity assay, or toxicity assessment, is set up in a multi-well format, with 96 or 384-well plates.

Next, cells are treated with drugs and processed for read-outs, which may include ATP assays, cell death assays, high-content imaging, or calcium oscillation.

4. Can you tell me a bit about how you apply 3D cell model workflows specifically to your research?

We are focusing on the development of automation protocols for automated cell cultures, as well as automated imaging and image analysis for complex 3D workflows. Recently, we developed and ran automated screening assays for finding more efficient anti-cancer drugs for triple-negative breast cancer. We used patient-derived cancer organoids representing a drug-resistant disease phenotype, and we applied automation to culture 3D organoids, simulate drug intervention, and run end-point assays for identifying the compounds that kill tumor cells. We tested a library of compounds and found several candidates that had greater efficacy than current standard drugs.

5. Can you explain how to automate the workflow for the development and analysis of organoids?

We created an automated workcell at Molecular Devices that combines several instruments in one complex system. It includes a Beckman Biomek automated liquid handler, a LiCONiC automated incubator, our ImageXpress HT.ai high-content imaging system, our SpectraMax plate reader and AquaMax washer, and a Bionex automated centrifuge. All the components are connected by a collaborative robot, PreciseFlex 400 that can move plates from one instrument to another at desired time points, while scheduling software ensures that all system elements work together seamlessly. Each instrument has multiple protocols designed for different steps, including feeding the cells and organoid plating, which can be called out by the scheduler at indicated times.

Organoid Innovation Center

The Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel 3D biology methods to address key challenges of scaling complex 3D biology.

Imaging methods are another exciting area of technology for organoid research. To image organoids or organ-on-a-chip, we need to use advanced optics. The ImageXpress high-content imaging system has several advantages for 3D samples:

Powerful lasers and confocal optics allow us to take the Z-stack of multiple images starting from the bottom and going up with steps like 5-10 microns Confocal optics allow us to reject the light that is out of focus so that we can get sharper images throughout organoids and Matrigel.

Next, our MetaXpress image analysis software analyzes the images in each 2D slice and converts data into 3D space. You can get multiple measurements to characterize organoids, cells, or subcellular organelles. These measurements help define cell counts, intensities, volumes, area, distances, and more, allowing us to monitor and quantitate changes in morphology, cell content, and viability. We also have machine learning elements, where users can train software to recognize objects, and features, to provide more efficient and insightful analysis.

6. How can automation aid in the research of complex systems specifically?

Automating would reduce labor and repetitive tasks like feeding cells every day or every second day for 2 months. Also, it will help to ramp up the research with higher throughput. For example, instead of studying 3 cell lines or 5 mutations, automation would allow you to test 50 cell lines to study 100 mutations.

High-content imaging powered by machine learning algorithms will allow to observe and characterize a variety of changes in organoids and cells, providing multiple readouts and yielding a valuable set of information about cell growth, differentiation, cell cycle, death, apoptosis, gene expression, or activation of signaling pathways.

7. How will you use these systems again in your future research?

In addition to cancer biology studies, we are actively developing other workflows, including but not limited to intestinal organoids, stem cell workflow, cardiac organoids, and more.

8. How will the automation of 3D organoid analysis evolve in the future?

We believe, as biology evolves and the complexity of assays increases, automation will be increasingly important for better understanding disease mechanisms, accelerating drug discovery, and eventually finding better ways to treat diseases.

By developing new and more advanced technologies and instruments, we believe we will further contribute to the progress of life sciences.

Understanding the basic principles behind 3D organoids - and the current bottlenecks - is crucial to the successful development and utilization of these advanced models for drug discovery. 

 

Listen to the full podcast

If you'd like to learn more, please enjoy the full podcast, “Complex assays with Ian Shoemaker, Beckman Coulter Life Sciences & Dr Oksana Sirenko, Molecular Device." 

 

Drug Target Review · Episode 14 - Ian Shoemaker, Beckman Coulter & Dr Oksana Sirenko, Molecular Devices

Here they discuss a comprehensive outlook on the science of organoid research – discover how organoid models are developed, used within our experts’ research, and how the automation of 3D organoid analysis will evolve in the future, plus much more!

 

  • Share on LinkedIn
  • Share on Facebook
  • Share on Twitter
  • Share on Youtube

Recent posts

View All Posts
Spacer

How 3D Cell Models Will Shape the Future of Drug Discovery

Target discovery and drug development rely heavily on 2D cell and animal…

A roundtable discussion
Spacer

A case study for assay-ready patient-derived organoids (PDOs) and high-throughput 3D imaging to advance drug discovery

Introduction – the problem.  The average cost of bringing a new drug to…

Read case study
Spacer

Celebrating the art of science: A D+I interview with Daniele Tortorella

At Molecular Devices, we cultivate a culture where associates around the…

Celebrate D+I
Spacer

Advanced technology for automated 3D biology workflows #SLASEurope2022

SLAS Europe 2022, hosted numerous sessions packed with the latest…

View SLAS EU activities
Spacer

[Podcast] Challenges of traditional cell line development and emerging technologies to help regulate monoclonality

Advancements in genetic engineering and synthetic biology have allowed…

Listen to CLD podcast
Compare /

MIKROPLATTEN-READER

  • SpectraMax i3x
  • SpectraMax iD3 / iD5
  • SpectraMax M Serie
  • SoftMax Pro Software

ZELLULÄRES IMAGING

  • ImageXpress Confocal HT.ai
  • ImageXpress Pico
  • IN Carta Bildanalyse-Software

KLON-SCREENING

  • ClonePix 2 Säugerzelllinie-Kolonie Picker
  • QPix Microbial Colony Picker

FLIPR-SYSTEM

AXON PATCH-CLAMP

LABOR-AUTOMATISIERUNG

GXP-KONFORMITÄT

  • Neuigkeiten
  • Ereignisse
2. Apr. 2023

Focus on Microscopy (FOM)

19. Apr. 2023

Solutions in Cell Line Development

9. Mai 2023

Imaging User Meeting 2023

Mehr Veranstaltungen

22. Feb. 2023

Silver Sponsor Molecular Devices at Society for Laboratory Automation and Screening 2023 International Conference and Exhibition

13. Feb. 2023

Molecular Devices inks deal with HUB Organoids to advance automated intestinal organoid screening technology

7. Feb. 2023

Molecular Devices partners with SEED Biosciences to exclusively offer DispenCell Single-Cell Dispenser, expanding leadership in cell line development

Mehr Neuigkeiten
Newsletter-Ladeanzeige
  • März 2023  
  • Februar 2023  
  • Januar 2023  

WELTWEITE HAUPTNIEDERLASSUNG

  • Anschrift-Icon

    Molecular Devices, LLC.
    3860 N First Street
    San Jose, CA 95134

  • Kontaktieren Sie uns

    Phone: +1 800-635-5577

    Phone: +86-400-821-3787

    Telefon: +44-118-944-8000

    Phone: +44-118-944-8000

    Phone: +82-2-3471-9531

    Teléfono: +44-118-944-8000

    Telefono: +44-118-944-8000

  • Bürozeiten

    Mon-Fri 8:00 am - 5:00 pm (PST)

    周一至周五,早上 9 点至下午 5 点 30 分

    Montag bis Freitag 8 h à 17 h (GMT)

    lundi à vendredi 8 h à 17 h (GMT)

    월~금, 오전 9시~ 오후 5시 (한국표준시)오후

    de lunes a viernes 8 h à 17 h (GMT)

    lunedi al venerdì 8 h à 17 h (GMT)

  • Produktvertrieb

    Sales +1 877-589-2214

    售前咨询 : 400-821-3787 转 1

    Verkauf 00800 665 32860

    Ventes 00800 665 32860

    매상 82 2 3471 9531

    Ventas 00800 665 32860

    Saldi 00800 665 32860

  • Technischer Kundendienst

    Support +1 800 635 5577

    售后咨询 : 400-821-3787 转 2

    Unterstützung +44-118-944-8000

    Support +44-118-944-8000

    지원하다 +82-2-3471-9531

    Apoyo +44-118-944-8000

    Supporto +44-118-944-8000

  • Wissensbasis

    Spectranet Kunden-Portal

Navigation Fußzeile

  • Allgemeine Geschäftsbedingungen
  • Datenschutz
  • Meine Daten nicht verkaufen oder teilen
  • Online-Nutzungsbedingungen
  • Warenzeichen
  • Support
  • Kontaktieren Sie uns
  • Sitemap
  • Danaher Life Sciences
  • Karriere

©2023 Molecular Devices, LLC. Alle Rechte vorbehalten.

©2023 Molecular Devices, LLC. 美谷分子仪器(上海)有限公司保留所有权利。
All Rights Reserved 沪ICP备05056171号-1

barcode