The human heart is a complex organ providing highly regulated processes of moving blood through the body. Recent publications show that tri-cellular co-culture microtissues of cardiomyocytes, endothelial cells and cardiac fibroblasts that are all derived from human iPSC enhance the maturation and functional activity of cells compared to 2D cardiomyocytes and thus more closely mimics actual heart physiology.
In this study, we used a tri-culture model created by mixing iPSC-derived cardiac cells with primary adult fibroblasts and iPSC-derived endothelial cells. A variety of parametric responses were demonstrated using a set of 8 known modulators of cardiac activities. The assay can be used to test developing drugs and screen chemicals for potential cardiotoxic hazard.